Brassica S-Proteins Accumulate in the Intercellular Matrix along the Path of Pollen Tubes in Transgenic Tobacco Pistils |
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| Authors: | Kandasamy M. K. Dwyer K. G. Paolillo D. J. Doney R. C. Nasrallah J. B. Nasrallah M. E. |
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| Affiliation: | Section of Plant Biology, Division of Biological Sciences, Cornell University, Ithaca, New York 14853. |
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| Abstract: | A tobacco plant transformed with a Brassica oleracea SLG-22 gene was analyzed by immunocytochemical methods to determine the localization of the transgene-encoded protein product. Immunolabeling was observed in the pistil along the path followed by pollen tubes after pollination. S-antigen accumulated in the intercellular matrix of the transmitting tissue of the style and its continuation in the basal portion of the stigma and outside a few special cells of the placental epidermis of the ovary. This pattern of S-antigen distribution closely resembles that described for the S-associated glycoproteins of self-incompatible Nicotiana alata and differs from its distribution in B. oleracea. |
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