Temperature sensitivity of the penicillin-induced autolysis mechanism in nongrowing cultures of Escherichia coli. |
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Authors: | W Kusser and E E Ishiguro |
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Abstract: | The effect of incubation temperature on the ampicillin-induced autolysis of nongrowing Escherichia coli was determined. The autolysis mechanisms in amino acid-deprived relA mutant cells treated with chloramphenicol were temperature sensitive. This temperature-sensitive autolysis was demonstrated in three independent ways: turbidimetric determinations, viable cell counts, and solubilization of radiolabeled peptidoglycan. |
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