Abscisic acid catabolism enhances dormancy release of grapevine buds |
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| Authors: | Chuanlin Zheng Atiako Kwame Acheampong Zhaowan Shi Amichay Mugzech Tamar Halaly‐Basha Felix Shaya Yufei Sun Violeta Colova Assaf Mosquna Ron Ophir David W. Galbraith Etti Or |
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| Affiliation: | 1. Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel;2. College of Life Sciences, South China Agricultural University, Guangzhou, China;3. The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel;4. Center for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A & M University, Tallahassee, Florida;5. School of Plant Sciences and BIO5 Institute, University of Arizona, Tucson, Arizona |
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| Abstract: | The molecular mechanism regulating dormancy release in grapevine buds is as yet unclear. It was formerly proposed that dormancy is maintained by abscisic acid (ABA)‐mediated repression of bud–meristem activity and that removal of this repression triggers dormancy release. It was also proposed that such removal of repression may be achieved via natural or artificial up‐regulation of VvA8H‐CYP707A4, which encodes ABA 8′‐hydroxylase, and is the most highly expressed paralog in grapevine buds. The current study further examines these assumptions, and its experiments reveal that (a) hypoxia and ethylene, stimuli of bud dormancy release, enhance expression of VvA8H‐CYP707A4 within grape buds, (b) the VvA8H‐CYP707A4 protein accumulates during the natural transition to the dormancy release stage, and (c) transgenic vines overexpressing VvA8H‐CYP707A4 exhibit increased ABA catabolism and significant enhancement of bud break in controlled and natural environments and longer basal summer laterals. The results suggest that VvA8H‐CYP707A4 functions as an ABA degrading enzyme, and are consistent with a model in which the VvA8H‐CYP707A4 level in the bud is up‐regulated by natural and artificial bud break stimuli, which leads to increased ABA degradation capacity, removal of endogenous ABA‐mediated repression, and enhanced regrowth. Interestingly, it also hints at sharing of regulatory steps between latent and lateral bud outgrowth. |
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| Keywords: | ABA 8′ ‐hydroxylase (ABA8'OH) |
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