Telomerase activity in germline and embryonic cells of Xenopus. |
| |
| Authors: | L L Mantell and C W Greider |
| |
| Affiliation: | Cold Spring Harbor Laboratory, NY 11724. |
| |
| Abstract: | Telomerase is a ribonucleoprotein which synthesizes telomere repeats onto chromosome ends. Telomerase activity is involved in telomere length maintenance. We used Xenopus laevis as a model system to study the expression of telomerase activity in germline cells and during early development. We identified a non-processive telomerase activity in manually dissected nuclei of Xenopus stage VI oocytes. Telomerase activity was detected throughout oogenesis and embryogenesis. Telomerase was active in both S and M phase cell cycle extracts, suggesting that telomerase activity is not regulated with chromosomal DNA replication. |
| |
| Keywords: | |
|
