Genetic Diversity of Fusarium oxysporum f.sp. lentis Population Affecting Lentil in Syria |
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| Authors: | Naiem Husien Al‐Husien Aladdin Hamwieh Seid Ahmed Bassam Bayaa |
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| Affiliation: | 1. General Commission of Scientific Agricultural Center of Aleppo, Aleppo, Syria;2. Biodiversity and Integrated Gene Management Program, ICARDA, Cairo, Egypt;3. Biodiversity and Integrated Gene Management Program, ICARDA, Rabat, Morocco;4. Department of Plant Protection, Faculty of Agriculture, University of Aleppo, Aleppo, Syria |
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| Abstract: | Lentil (Lens culinaris Medik.) is an important food legume crop in Syria. Fusarium wilt (Fusarium oxysporum f.sp. lentis – Fol) is a key yield‐limiting factor in the country. The genetic diversity of Fol population was studied using 96 isolates collected from different parts of the country using molecular markers. A total of 16 markers, random amplified polymorphic DNA, simple sequence repeats and inter‐simple sequence repeats were used and 218 polymorphic markers (scorable bands) were obtained. Cluster and structure analyses grouped the isolates into three major groups and subgroups indicating high genetic diversity in the pathogen populations. The molecular variance within the population accounted 87% of the total variation indicating high diversity within population than among geographic locations. The result of this study showed that no alleles were linked to specific province, and therefore, screening for the Fusarium wilt in one location using virulent isolates could be enough to save time and resources. |
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| Keywords: | Fusarium wilt
ISSR
RAPD
SSR
vascular disease |
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