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| 巴氏新小绥螨毒死蜱抗性选育及生物学特性 | |
| 引用本文： | 王莉,程明明,傅云梅,方云洪,魏志堂,成禄艳,雷双,丁莉莉,于士将,丛林,冉春. 巴氏新小绥螨毒死蜱抗性选育及生物学特性[J]. 昆虫学报, 2021, 64(3): 374-383. DOI: 10.16380/j.kcxb.2021.03.009 |
| 作者姓名： | 王莉 程明明 傅云梅 方云洪 魏志堂 成禄艳 雷双 丁莉莉 于士将 丛林 冉春 |
| 作者单位： | (西南大学柑桔研究所, 重庆 400712) |
| 基金项目： | 国家重点研发计划(2018YFD0201508,2019YFD1002100,2020YFD10001020);重庆市技术创新与应用发展专项(cstc2019jscx-gksb0218);重庆市科技创新领军人才支持计划(cstc2018kjcxljrc0040);重庆市自然科学基金项目(cstc2020jcyj-msxmX0980)。 |
| 摘 要： | [目的]明确巴氏新小绥螨Neoseiulus barkeri毒死蜱抗性对其生物学特性的影响,为抗药性捕食螨田间应用提供理论基础.[方法]在室内采用药膜法测定毒死蜱对巴氏新小绥螨的毒力,以半致死浓度(lethal medium concentration,LC50)为选择压力,对巴氏新小绥螨敏感种群进行逐代汰选.应用生命... |
| 关 键 词： | 巴氏新小绥螨 毒死蜱 抗药性 生殖力 捕食功能反应 生物防治 |
Resistance selection and biological characteristics of Neoseiulus barkeri (Acari: Phytoseiidae) to chlorpyrifos |
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| WANG Li,CHENG Ming-Ming,FU Yun-Mei,FANG Yun-Hong,WEI Zhi-Tang,CHENG Lu-Yan,LEI Shuang,DING Li-Li,YU Shi-Jiang,CONG Lin,RAN Chun. Resistance selection and biological characteristics of Neoseiulus barkeri (Acari: Phytoseiidae) to chlorpyrifos[J]. Acta Entomologica Sinica, 2021, 64(3): 374-383. DOI: 10.16380/j.kcxb.2021.03.009 | |
| Authors: | WANG Li CHENG Ming-Ming FU Yun-Mei FANG Yun-Hong WEI Zhi-Tang CHENG Lu-Yan LEI Shuang DING Li-Li YU Shi-Jiang CONG Lin RAN Chun |
| Affiliation: | (Citrus Research Institute, Southwest University, Chongqing 400712, China) |
| Abstract: | 【Aim】 To clarify the effects of acquired chlorpyrifos resistance in the predatory mite Neoseiulus barkeri on its biological characteristics, so as to provide a theoretical basis for its field application. 【Methods】 The residual film method was adopted to test the toxicity of chlorpyrifos to N. barkeri in the laboratory, and the lethal medium concentration (LC50) was chosen as the selection pressure. Life table was used to assess the effects of acquired chlorpyrifos resistance on the relative fitness (Rf)of N. barkeri. In addition, Holling type II model was used to analyze the differences in the predation functional response of the chlorpyrifos-susceptible and resistant strains of N. barkeri to eggs and female adults of Panonychus citri at different temperatures.【Results】 A chlorpyrifos-resistant strain of N. barkeri with the resistance ratio of 34.77-fold was obtained after selection of 21 generations. The acquired resistance had no significant effects on the developmental duration, proportion of female offspring and predation functional response of N. barkeri, but shortened the female adult longevity and oviposition period. The susceptible and resistant strains of N. barkeri could not complete the whole generation at 15℃. The fecundity (number of eggs laid per female) of the susceptible and resistant strains of N. barkeri was the highest at 25℃, and the fecundity was significantly higher for the susceptible strain (41.64±1.04 eggs/female) than for the resistant strain (38.80±0.93 eggs/female). Likewise the oviposition periods of the susceptible and resistant strains of N. barkeri were the longest at 25℃, and the oviposition period was significantly longer for the susceptible strain (24.82±1.50 d) than for the resistant strain (21.34±1.26 d). Further, the shortest developmental duration of the susceptible and resistant strains of N. barkeri was recorded at 30℃, being 6.62±0.23 d for the susceptible strain and 6.53±0.13 d for the resistant strain. Meanwhile, the strongest predation ability of the susceptible and resistant strains of N. barkeri to eggs or female adults of P. citri was recorded at 30℃, with the daily maximum predation amounts of the susceptible and resistant strains of N. barkeri to P. citri eggs being 156.25 and 140.85, respectively, and to female adults of P. citri being 23.10 and 22.32, respectively. The relative fitness (Rf) of the resistant strain of N. barkeri was lower than that of the susceptible strain at 25 and 30℃, but slightly higher than that of the susceptible strain at 20 and 35℃. 【Conclusion】 Generally, chlorpyrifos resistance in N. barkeri has little effect on its biological characteristics under different temperatures. The study results provide a theoretical basis for screening resistant strains of N. barkeri and their field application. |
| Keywords: | Neoseiulus barkeri chlorpyrifos insecticide resistance fecundity predation functional response biological control |
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