Some effects of chlorambucil on enzymes of glutathione metabolism in drug-sensitive and -resistant strains of the Yoshida ascites sarcoma |
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| Authors: | K. R. Harrap R. C. Jackson Bridget T. Hill |
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| Affiliation: | Chester Beatty Research Institute, Institute of Cancer Research: Royal Cancer Hospital, London, S.W. 3 |
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| Abstract: | 1. Yoshida ascites cells from a strain sensitive to chemotherapy with alkylating agents contained elevated activities of the two enzymes directly responsible for glutathione synthesis, in comparison with a resistant cell strain. The activities of the glutathione-degrading enzyme γ-glutamyltranspeptidase were comparable in both cell strains. 2. After parenteral administration of chlorambucil to rats carrying either strain of tumour, the activities of the glutathione-synthetic enzymes increased in the sensitive cells, but remained unchanged in the resistant cells. Drug treatment was without effect on the γ-glutamyltranspeptidase activity of either cell strain. 3. The activities of a number of enzymes concerned in the oxidoreduction of glutathione remained unchanged after the administration of chlorambucil to rats carrying either strain of cells. |
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