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Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides. |
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| Authors: | T D rper E L Winnacker |
| Affiliation: | T Dörper and E L Winnacker |
| Abstract: | The paper describes an improved method for the synthesis of oligodeoxyribonucleotides using phosphoramidite chemistry. Our procedure relies on novel phosphoramidite intermediates, the deoxyribonucleoside-3'-morpholine-methoxyphosphins. These compounds are extremely stable and can be purified readily. Condensation reactions during solid-phase synthesis can thus be performed with high efficiency and result in a high yield synthesis of long chain oligodeoxyribonucleotides. |
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