| 褐飞虱和桃蚜粗提液中酚氧化酶原激活系统的激活特点 |
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| 引用本文： | 陈娇,徐均焕,冯明光. 褐飞虱和桃蚜粗提液中酚氧化酶原激活系统的激活特点[J]. 昆虫学报, 2006, 49(2): 194-199 |
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| 作者姓名： | 陈娇 徐均焕 冯明光 |
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| 作者单位： | 浙江大学生命科学学院微生物研究所，杭州 310029 |
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| 基金项目： | 科技部科技攻关项目西部开发科技行动;中国科学院资助项目;浙江省自然科学基金 |
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| 摘 要： | 采用研磨法制备褐飞虱Nilaparvata lugens (Stål)和桃蚜Myzus persicae (Sulzer)粗提液，用于研究几种化学因子对粗提液中酚氧化酶原激活系统(proPO-AS)的激活特点。结果表明, 在0.1～100 mmol/L的Ca2+浓度范围内，虱液和蚜液的酚氧化酶(PO)活性随Ca2+浓度升高而增强，并在30 mmol/L处PO活性达到最高，在此限之上PO活性反而下降。当昆布多糖浓度从10-4 mg/mL升至10 mg/mL时，虱液和蚜液的PO活性也随之升高，但再提高昆布多糖浓度却未见PO活性的峰顶出现。在6种葡聚糖对PO的激活作用中，昆布多糖和酵母聚糖能显著增强PO活性，但curdlan对虱液和蚜液的PO无明显激活作用，而甘露聚糖、右旋糖苷和纤维素则降低PO活性。这些结果显示β-1,3-葡聚糖能有效激活proPO-AS。Ca2+和丝氨酸蛋白酶抑制剂PMSF加样顺序的不同也会影响PO活性。
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| 关 键 词： | 褐飞虱 桃蚜 酚氧化酶原激活系统 酚氧化酶 昆虫免疫 |
| 文章编号： | 0454-6296(2006)02-0194-06 |
| 收稿时间： | 2005-07-12 |
| 修稿时间： | 2005-11-25 |
Features of the prophenoloxidase-activating system in crude extracts of Nilaparvata lugens and Myzus persicae |
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| CHEN Jiao,XU Jun-Huan,FENG Ming-Guang. Features of the prophenoloxidase-activating system in crude extracts of Nilaparvata lugens and Myzus persicae[J]. Acta Entomologica Sinica, 2006, 49(2): 194-199 |
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| Authors: | CHEN Jiao XU Jun-Huan FENG Ming-Guang |
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| Affiliation: | Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310029, China |
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| Abstract: | The prophenoloxidase (proPO) activating system (proPO-AS) involved in crude extracts of rice planthopper Nilaparvata lugens (Stal) and green peach aphid Myzus persicae (Sulzer) was investigated in relation to potentially influential agents. The results showed that as Ca2+ concentration increased from 0.1 mmol/L to 100 mmol/L, the activities of phenoloxidase (PO) in the crude extracts of N. lugens and M. persicae were concentration dependent. The activation of the proPO peaked at 30 mmol/L of the Ca2+ concentration. However, a further increase of the Ca2+ to 100 mmol/L resulted in a decrease in proPO activation. Incubation of the extracts in conjunction with different concentrations of laminarin (from 10-4 mg/mL to 10 mg/mL) witnessed the levels of proPO activation entirely depending on the laminarin concentrations. When different glucans were used as activating agents, laminarin and zysoman significantly enhanced the PO activities whereas the effect of curdlan was not significant compared to a blank control. In contrast, the PO activities were reduced when mannan, dextran or cellulose was added. These results indicated that the β-1,3-glucan effectively activated the proPO present in the extracts of the two insect species with the proPO-AS selectively responding to different glucans. The sequence of adding Ca2+ and phenylmethyl sulfonyl fluorid (a serine protease inhibitor) in the extracts also affected the PO activities. |
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| Keywords: | Nilaparvata lugens Myzus persicae prophenoloxidase-activating system prophenoloxidase insect immunity |
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