| 桦木酸对胃癌SGC-7901细胞凋亡的影响* |
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| 引用本文： | 丁璐,邵淑丽,何孟奇,黄鑫,张伟伟,张珍珠. 桦木酸对胃癌SGC-7901细胞凋亡的影响*[J]. 中国应用生理学杂志, 2021, 37(5): 495-499. DOI: 10.12047/j.cjap.6091.2021.060 |
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| 作者姓名： | 丁璐 邵淑丽 何孟奇 黄鑫 张伟伟 张珍珠 |
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| 作者单位： | 1.齐齐哈尔大学生命科学与农林学院; 2.抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 黑龙江 齐齐哈尔 161006 |
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| 基金项目： | *齐齐哈尔大学黑龙江省教育厅基本业务专项重点项目(135109104);黑龙江省高教强省优势特色学科—玉米“粮头食尾”重点专项(LTSW201737);黑龙江省省属高等学校基本科研业务费科研项目(植物性食品加工技术特色学科专项 YSTSXK201809) |
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| 摘 要： | 目的: 以人胃癌SGC-7901细胞为研究对象,探究桦木酸对其凋亡的影响。方法: 将人胃癌SGC-7901细胞分为4组,每组设置3个复孔,对照组未加入桦木酸,而三组实验组分别加入浓度为10 mg/L、20 mg/L及30 mg/L的桦木酸,将各组细胞放入5%的CO2培养箱中培养48 h,激光共聚焦显微镜观察细胞形态变化;流式细胞术检测细胞凋亡率和线粒体膜电位变化;qRT-PCR和Western blot分别检测SGC-7901细胞凋亡相关基因Bcl-2、Bax和Caspase-3在mRNA和蛋白水平的表达。结果: 与对照组相比,终浓度为10 mg/L、20 mg/L、30 mg/L的桦木酸处理组,细胞发生皱缩、细胞核裂解并出现凋亡小体;细胞早期凋亡与晚期凋亡率显著增加(P＜0.05 or P＜0.01),线粒体膜电位明显降低(P＜0.05 or P＜0.01);细胞凋亡相关基因Bax和Caspase-3的mRNA与蛋白表达水平均显著上升(P＜0.01),而Bcl-2的mRNA与蛋白表达水平显著降低(P＜0.01)。结论: 在一定浓度范围内,桦木酸通过调节凋亡相关基因Bcl-2、Bax和Caspase-3的表达诱导人胃癌SGC-7901细胞凋亡。
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| 关 键 词： | 桦木酸 人胃癌SGC-7901细胞 细胞凋亡 |
| 收稿时间： | 2020-05-14 |
Effects of Betulinic acid on apoptosis in human gastric cancer SGC-7901 cells |
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| DING Lu,SHAO Shu-li,HE Meng-qi,HUANG Xin,ZHANG Wei-wei,ZHANG Zhen-zhu. Effects of Betulinic acid on apoptosis in human gastric cancer SGC-7901 cells[J]. Chinese journal of applied physiology, 2021, 37(5): 495-499. DOI: 10.12047/j.cjap.6091.2021.060 |
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| Authors: | DING Lu SHAO Shu-li HE Meng-qi HUANG Xin ZHANG Wei-wei ZHANG Zhen-zhu |
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| Affiliation: | 1. College of Life Science and Agriculture and Forestry; 2. Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University, Qiqihaer 161000, China |
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| Abstract: | Objective: To investigate the effects of betulinic acid on apoptosis of human gastric cancer SGC-7901 cells. Methods: The human gastric cancer SGC-7901cells were divided in to 4 groups, and each group was set with 3 replicates. The SGC-7901cells in control group were not treated with betulinic acid; the other 3 experimental groups were treated with betulinic acid at the concentrations of 10, 20 and 30 mg/L, respectively; each group was incubated in a 5% carbon dioxide incubator for 48 h. Laser confocal microscope was used to observe morphological changes of SGC-7901 cells; Flow cytometry was applied to determine apoptosis rate and mitochondrial membrane potential. The mRNA and protein levels of Bcl-2, Bax and Caspase-3 were also detected by qRT-PCR and western blot respectively. Results: Compared with the control group, SGC-7901 cells in the treated group at final concentrations of 10, 20 and 30 mg/L shrinked, appeared apoptosis body along with nuclear splitting. The percentage of cells in early and advanced period of apoptosis were markedly increased (P＜0.05 or P＜0.01), mitochondrial membrane potential was obviously reduced (P＜0.05 or P＜0.01). qRT-PCR and western blot analysis showed that the mRNA and protein expressions of Bax and Caspase-3 were increased significantly (P＜0.01), while the expressions of Bcl-2 were decreased significantly (P＜0.01). Conclusion: Within a certain range of concentrations, betulinic acid induces cell apoptosis by regulating the expression of Bcl-2, Bax and Caspase-3 in human gastric cancer. |
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| Keywords: | betulinic acid human gastric carcinoma SGC-7901 cells cell apoptosis |
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