Suppression of a Mutation in Gene 3 of Bacteriophage T7 (T7 Endonuclease I) by Mutations in Phage and Host Polynucleotide Ligase |
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| Authors: | Paul D. Sadowski |
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| Affiliation: | Division of Experimental Pathology, Department of Pathology, University of Toronto, Toronto, Canada |
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| Abstract: | Bacteriophage T7 bearing amber mutations in both gene 1.3 (T7 DNA ligase) and gene 3 (T7 endonuclease I) are viable when grown in suppressor-negative, ligase-negative hosts. This is evidenced by a high plating efficiency and a large burst size compared to the single mutants. These findings may be explained by a limited destruction of cellular DNA by the double mutant. |
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