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Nitrogen Fixation in Continuous Culture with NH4Cl-Containing Media
Authors:C. Fritzsche and E.-G. Niemann
Abstract:N balance and 15N dilution were determined from growth of Azospirillum brasilense Sp7 and two unidentified gram-negative nitrogen-fixing microorganisms in continuous culture supplied with NH4Cl. At the 1.1 and 2.2 mM NH4Cl steady states (N-to-C ratios of 1:68 and 1:34, respectively), the organisms grew with NH4Cl and N2 as N sources simultaneously under carbon limitation. No ammonium could be detected in the supernatant of these cultures.
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