Separation of Replicative Intermediate from Single-stranded Ribonucleic Acid by Sedimentation at Low Ionic Strength |
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| Authors: | M. L. Fenwick |
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| Affiliation: | Dunn School of Pathology, University of Oxford, England |
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| Abstract: | By sedimentation in 2.5 x 10(-4)m sodium ethylenediaminetetraacetate at 25 C, replicative intermediate could be separated from single-stranded ribonucleic acid, both viral and ribosomal. All of the label in the replicative intermediate after a brief pulse of titrated uridine was resistant to ribonuclease. |
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