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腐食酪螨螨体正己烷提取物的生物活性及成分分析
引用本文:刘婷,金道超. 腐食酪螨螨体正己烷提取物的生物活性及成分分析[J]. 昆虫学报, 2012, 55(12): 1413-1417
作者姓名:刘婷  金道超
作者单位:1. 贵州大学昆虫研究所,贵阳550025;皖南医学院医学寄生虫学教研室,安徽芜湖241000
2. 贵州大学昆虫研究所,贵阳,550025
基金项目:农业部农作物病虫鼠害疫情监测与防治项目(20130108);贵州省科技创新人才基地-农林生物灾害防治人才基地专项(黔人领发〔2011〕1);高等学校省级优秀青年人才基金(2012SQRL125);皖南医学院中青年科研基金(WK201114)
摘    要:【目的】确定腐食酪螨Tyrophagus putrescentiae报警信息素的存在及其成分。【方法】采取正己烷溶剂浸提法提取获得腐食酪螨螨体提取物, 通过观察腐食酪螨对提取物的行为反应确定其生物活性, 最后用气相色谱-质谱联用仪(gas chromatograph-mass spectrum, GC-MS)对提取物的成分进行分析。【结果】腐食酪螨正己烷提取物对该螨有明显的报警作用。GC-MS分析显示, 提取物中含有橙花醇甲酸酯、Z-柠檬醛、E-柠檬醛等成分。【结论】该实验证明了腐食酪螨报警信息素的存在, 为进一步明确各成分及其作用奠定了基础。

关 键 词:腐食酪螨   报警信息素   行为反应   橙花醇甲酸酯   Z-柠檬醛   E-柠檬醛  

Biological activity and chemical composition analysis of hexane extracts from Tyrophagus putrescentiae (Acari: Acaridae)
LIU Ting,JIN Dao-Chao. Biological activity and chemical composition analysis of hexane extracts from Tyrophagus putrescentiae (Acari: Acaridae)[J]. Acta Entomologica Sinica, 2012, 55(12): 1413-1417
Authors:LIU Ting  JIN Dao-Chao
Affiliation:1,* (1. Institution of Entomology, Guizhou University, Guiyang 550025, China; 2. Department of Parasitology, Wannan Medical College, Wuhu, Anhui 241000, China)
Abstract:【Aim】 To explore the components of the alarm pheromone of Tyrophagus putrescentiae. 【Methods】 The extracts were extracted from T. putrescentiae mites with hexane. Biological activity of the extracts was assayed by observing the behavioral response of the mite to the extracts. The components of the extracts were identified by GC-MS. 【Results】 T. putrescentiae had intensive alarm response to the extracts, which consist of neryl formate, Z-citral, and E-citral based on GC-MS data. 【Conclusion】 This experiment proved the existence of alarm pheromone in T. putrescentiae and established the foundation for its further research.
Keywords:Tyrophagus putrescentiae')"   href="  #"  >Tyrophagus putrescentiae  alarm pheromone  behavioral response  neryl formate  Z-citral')"   href="  #"  >Z-citral  E-citral')"   href="  #"  >E-citral
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