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Plasmid visualization and nif gene location in nitrogen-fixing Azospirillum strains. |
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| Authors: | J Plazinski P J Dart B G Rolfe |
| Abstract: | A modified gel electrophoresis technique provided a reproducible way of detecting and isolating plasmids with molecular weights ranging from 12 X 10(6) to 370 X 10(6) for Azospirillum species. Analysis with the nifHD region of Rhizobium trifolii showed that the Azospirillum nif genes were chromosomally located in all eight strains investigated and not on endogenous plasmids. |
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