AFM visualization of mobile influenza A M2 molecules in planar bilayers |
| |
| Authors: | Hughes Travis Strongin Bradley Gao Fei Philip Vijayvergiya Viksita Busath David D Davis Robert C |
| |
| Affiliation: | Department of Physics and Astronomy, Brigham Young University, Provo, Utah, USA. Travis.Hughes@colorado.edu |
| |
| Abstract: | We report the observation of influenza A M2 (M2) incorporated in a dipalmitoylphosphatidylcholine (DPPC) supported planar bilayer on mica, formed by use of a modified vesicle fusion method from proteoliposomes and visualized with contact mode atomic force microscopy. Incubation of proteoliposomes in a hyperosmotic solution and increased DPPC/M2 weight ratios improved supported planar bilayer formation by M2/DPPC proteoliposomes. M2's extra-bilayer domains were observed as particles estimated to protrude 1-1.5 nm above the bilayer surface and <4 nm in diameter. Particle density was 5-18% of the nominal tetramer density. Movement of observable M2 particles was independent of the probe tip. The mean lateral diffusion coefficient (D) of M2 was 4.4 +/- 1.0 x 10(-14) cm(2)/s. Eighty-two percent of observable particles were mobile on the observable timescale (D > 6 x 10(-15) cm(2)/s). Protein-protein interactions were also observed directly. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
