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Pichia acaciae Killer System: Genetic Analysis of Toxin Immunity |
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| Authors: | John P. Paluszynski Roland Klassen Friedhelm Meinhardt |
| Abstract: | The gene responsible for self-protection in the Pichia acaciae killer plasmid system was identified by heterologous expression in Saccharomyces cerevisiae. Resistance profiling and conditional toxin/immunity coexpression analysis revealed dose-independent protection by pPac1-2 ORF4 and intracellular interference with toxin function, suggesting toxin reinternalization in immune killer cells. |
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