| 斜纹夜蛾两个天蚕素D基因的克隆及序列分析 |
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| 引用本文： | 陈维春,宋杰,庞义. 斜纹夜蛾两个天蚕素D基因的克隆及序列分析[J]. 昆虫学报, 2007, 50(7): 745-749 |
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| 作者姓名： | 陈维春 宋杰 庞义 |
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| 作者单位： | (中山大学有害生物控制与资源利用国家重点实验室，昆虫学研究所，广州 510275) |
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| 摘 要： | 天蚕素是昆虫抵御病菌入侵的一类抗菌肽家族。根据斜纹夜蛾Spodoptera litura天蚕素B基因设计特异引物，通过PCR扩增得到2个新的天蚕素基因部分序列，分别命名为cecD1和cecD2（GenBank登录号分别为EF555567和EF555568）。2个基因编码同一个天蚕素D蛋白，该蛋白的成熟肽与天蚕素B存在2个氨基酸残基差异。序列分析发现cecD1和cecD2中分别包含568 bp和377 bp的内含子序列，它们有相同的5′和3′拼接位点，A+T含量分别为59.7%和69.8%，符合大多数真核生物内含子高A+T含量的特征。
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| 关 键 词： | 斜纹夜蛾 抗菌肽 天蚕素 内含子 基因结构 |
| 文章编号： | 0454-6296（2007）07-0745-05 |
| 收稿时间： | 2007-02-15 |
| 修稿时间： | 2007-04-27 |
Cloning and sequence analysis of two cecropin D genes from the common cutworm, Spodoptera litura ( Lepidoptera: Noctuidae) |
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| CHEN Wei-Chun,SONG Jie,PANG Yi. Cloning and sequence analysis of two cecropin D genes from the common cutworm, Spodoptera litura ( Lepidoptera: Noctuidae)[J]. Acta Entomologica Sinica, 2007, 50(7): 745-749 |
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| Authors: | CHEN Wei-Chun SONG Jie PANG Yi |
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| Affiliation: | (State Key Laboratory for Biocontrol, Institute of Entomology, Sun Yat-Sen University, Guangzhou 510275, China) |
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| Abstract: | Cecropins are a family of antibacterial peptides synthesized in insects as a response to a bacterial infection. Two primers were designed according to cecropin B gene from Spodoptera litura and were used to PCR. As the result, two new cecropin genes, named cecD1 and cecD, were obtained, which encoded identical preprocecropin D. There was 2 amino acid residues difference between the mature peptide of preprocecropin D and that of cecropin B from S. litura. Two introns, 568 bp and 377 bp long, were found in cecD1 and cecD2, respectively, both having the same 5′and 3′splicing sites. The A+T content was 59.7% in cecD1 intron and 69.8% in cecD2 intron, conforming to the high A+T content characteristics of most eukaryotic introns. |
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| Keywords: | Spodoptera litura antibacterial peptide cecropin intron gene structure |
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