| 金线莲HPLC指纹图谱研究 |
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| 引用本文： | 李轶奕,刘顺治,谢婷婷,范世明,丘鹰昆. 金线莲HPLC指纹图谱研究[J]. 亚热带植物科学, 2021, 50(4): 267-271. DOI: 10.3969/j.issn.1009-7791.2021.04.005 |
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| 作者姓名： | 李轶奕 刘顺治 谢婷婷 范世明 丘鹰昆 |
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| 作者单位： | (1.厦门大学，福建 厦门 361105；2.福建中医药大学，福建 福州 350122) |
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| 基金项目： | 第四次全国中药资源普查项目(财社[2018]43 号) |
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| 摘 要： | 以14批不同来源金线莲药材为原料，采用高效液相色谱法(HPLC)，乙腈-甲酸水溶液为流动相，梯度洗脱，建立栽培及野生金线莲(Anoectochilus roxburgii)的指纹图谱，结合相似度分析，探讨金线莲与银线莲(Goodyera hachijoensis)的差异。结果表明，金线莲HPLC指纹图谱标定了24个共有峰，并指认了金线莲苷和葫芦巴碱、鼠李素-3-O-芸香糖苷、槲皮素、山柰酚、异鼠李素、Batatasin-Ⅲ等6个共有峰。除3批药材外，其余11批相似度均高于0.910，相似度总体较好。银线莲图谱相似度仅达0.529、0.721、0.698，均低于14批金线莲样品间的相似度。根据相似度评价，认为金线莲与银线莲存在差异，该结果可为分析评价金线莲与银线莲差异提供参考。
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| 关 键 词： | 金线莲 银线莲 HPLC指纹图谱 相似度评价 栽培 |
| 收稿时间： | 2021-04-26 |
| 修稿时间： | 2021-06-29 |
HPLC Fingerprinting of Anoectochilus roxburgii |
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| LI Yi-yi,LIU Shun-zhi,XIE Ting-ting,FAN Shi-ming,QIU Ying-kun. HPLC Fingerprinting of Anoectochilus roxburgii[J]. Subtropical Plant Science, 2021, 50(4): 267-271. DOI: 10.3969/j.issn.1009-7791.2021.04.005 |
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| Authors: | LI Yi-yi LIU Shun-zhi XIE Ting-ting FAN Shi-ming QIU Ying-kun |
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| Affiliation: | (1.Xiamen University, Xiamen 361105, Fujian China; 2.Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian China) |
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| Abstract: | In this experiment, the fingerprints of common cultivated and wild Anoectochilus roxburgii including 14 batches of herbs from different sources were established by high performance liquid chromatography (HPLC) with gradient elution using acetonitrile-formic acid aqueous solution as the mobile phase. The results showed that the HPLC fingerprint of A. roxburgii confirmed 24 common peaks including 6 common peaks of kinsenoside and trigonelline, rhamnetin-3-O-rutinoside, quercetin, kaempferol, isorhamnetin and batatasin-III. Except for 3 batches of medicinal materials, the similarity of the other 11 batches was higher than 0.910, and the similarity was generally good. The similarity of Goodyera hachijoensis only reached 0.529, 0.721, and 0.698, all of which were lower than the similarity within 14 batches of A. roxburgi. Based on the similarity evaluation, it was concluded that differences existed between A. roxburgii and G. hachijoensis. The results provide a reference for the analysis and evaluation of the differences between A. roxburgii and G. hachijoensis. |
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| Keywords: | Anoectochilus roxburgii Goodyera hachijoensis HPLC fingerprint similarity evaluation cultivation |
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