| 间歇运动对心梗大鼠心肌氧化应激和炎症的影响及其机制 |
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| 引用本文： | 耿元文,林琴琴,王湘怡,李若明,田振军. 间歇运动对心梗大鼠心肌氧化应激和炎症的影响及其机制[J]. 中国应用生理学杂志, 2021, 37(4): 439-444. DOI: 10.12047/j.cjap.6094.2021.043 |
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| 作者姓名： | 耿元文 林琴琴 王湘怡 李若明 田振军 |
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| 作者单位： | 1. 燕山大学 体育学院, 河北 秦皇岛 066004;2. 陕西师范大学 体育学院, 西安 710062 |
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| 基金项目： | *国家自然科学基金项目(31300978);河北省自然科学基金(C2019203537);河北省高等学校科学技术研究项目(QN2019068) |
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| 摘 要： | 目的:探讨间歇运动激活心肌梗死(MI)大鼠SIRT1-Nox4-ROS通路抑制心脏氧化应激和炎症的作用.方法:选取雄性SD大鼠30只,随机分为假手术对照(C)组,心肌梗死(MI)组和心梗+间歇运动(ME)组,每组10只.MI组采用心脏左冠状动脉前降支(LAD)结扎法,建立MI模型.C组大鼠实施假手术,ME组大鼠在MI手...
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| 关 键 词： | 心肌梗死 间歇运动 SIRT1 氧化应激 炎症 大鼠 |
Effects of aerobic interval training on myocardial oxidative stress and inflammation in rats with myocardial infarction and its mechanism |
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| GENG Yuan-wen,LIN Qin-qin,WANG Xiang-yi,LI Ruo-ming,TIAN Zhen-jun. Effects of aerobic interval training on myocardial oxidative stress and inflammation in rats with myocardial infarction and its mechanism[J]. Chinese journal of applied physiology, 2021, 37(4): 439-444. DOI: 10.12047/j.cjap.6094.2021.043 |
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| Authors: | GENG Yuan-wen LIN Qin-qin WANG Xiang-yi LI Ruo-ming TIAN Zhen-jun |
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| Affiliation: | 1. Yanshan University, Qinhuangdao 066004;2. Shaanxi Normal University, Xi'an 710062, China |
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| Abstract: | Objective: The present study was to determine the effects of aerobic interval training (AIT) on the expressions of SIRT1, Nox4 and inflammatory factor in the heart of rats with myocardial infarction (MI). Methods: Male Sprague Dawley rats were randomly divided into sham-operated group (C), sedentary MI group (MI) and MI with AIT group (ME) (n=10). The MI model was established by ligation the left anterior descending coronary artery. Rats in C groups were subjected to the same surgery, but only threaded and not ligated. After surgery 1 week, rats in ME groups took adaptability training for 1 week, and then subjected to 4 weeks treadmill exercise training. After training, the hearts were collected for histological observation. The level of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in heart was assessed by ultraviolet spectrophotometry. The activity of lactate dehydrogenase (LDH) was determined by enzyme linked immunosorbent assay. The expression of sirtuin1 (SIRT1) mRNA was examined by real-time quantitative polymerase chain reaction (RT-qPCR). The protein expressions of SIRT1, NADPH oxidase 4 (Nox4), tumor necrosis factor-alpha (TNF-α) and interleukin-1β (IL-1β) were detected by Western blotting. The levels of reactive oxygen species (ROS) were detected by dihydroethidium (DHE) staining. Results: Compared with the C group, the expression level of cardiac Nox4 protein was increased (P＜0.01), the level of MDA, activity of LDH and the level of ROS were increased significantly (P＜0.01), and the expressions of TNF-α and IL-1β protein were augmented in the heart of rats with MI (P＜0.01). However, the expressions of SIRT1 mRNA and protein and the activity of SOD were obviously decreased in MI group (P＜0.01). Furthermore, compared with the MI group, AIT increased the expressions of SIRT1 mRNA and protein and the activity of SOD in the heart of ME group (P＜0.01); Meanwhile, the expressions of cardiac Nox4, MDA level, LDH activity and ROS level were diminished in ME group (P＜0.01) as well as the decreased expressions of TNF-α and IL-1β protein (P＜0.01). SIRT1 expression was negatively related to the expressions of NOX4 and ROS. Conclusion: AIT obviously inhibited myocardial oxidative stress and inflammatory reaction, improved cardiac function in rats with MI, and the mechanism was closely related to the activation of SIRT1-Nox4-ROS signaling pathway. |
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| Keywords: | myocardial infarction aerobic interval training SIRT1 oxidative stress inflammation rats |
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