| 白介素11拮抗剂对实验性小鼠肺纤维化的作用 |
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| 引用本文： | 吴礼循,吴翔. 白介素11拮抗剂对实验性小鼠肺纤维化的作用[J]. 中国应用生理学杂志, 2021, 37(3): 308-312. DOI: 10.12047/j.cjap.6047.2021.015 |
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| 作者姓名： | 吴礼循 吴翔 |
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| 作者单位： | 1. 海口市妇幼保健院检验科; 2. 海南省中医院检验科, 海口 570203 |
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| 摘 要： | 目的:观察拮抗白介素11(IL-11)对博来霉素(BLM)诱导的实验性小鼠肺纤维化的作用.方法:将120只雄性C57BL/6小鼠随机分为正常对照组、IL-11拮抗剂组、BLM组和BLM+IL-11拮抗剂组(每组各30只).BLM组和BLM+IL-11拮抗剂组小鼠一次性气管注射BLM(1.5 mg/kg)诱导肺纤维化.于...
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| 关 键 词： | 肺纤维化 白介素11 博来霉素 胶原沉积 小鼠 |
Effects of interleukin-11 antagonist on pulmonary fibrosis in mice |
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| WU Li-xun,WU Xiang. Effects of interleukin-11 antagonist on pulmonary fibrosis in mice[J]. Chinese journal of applied physiology, 2021, 37(3): 308-312. DOI: 10.12047/j.cjap.6047.2021.015 |
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| Authors: | WU Li-xun WU Xiang |
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| Affiliation: | 1. Department of Clinical Laboratory, Haikou Hospital of the Maternal and Child Health;2. Department of Clinical Laboratory, Hainan Provincial Hospital of Traditional Chinese Medicine, Haikou 570203, China |
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| Abstract: | Objective: To investigate the effects of interleukin 11 (IL-11) antagonist on bleomycin (BLM)-induced pulmonary fibrosis in mice. Methods: C57BL/6 mice were randomly divided into the control group, IL-11 antagonist group, BLM group, and BLM + IL-11 antagonist group (30 in each group). Mice in the BLM group and BLM + IL-11 antagonist group were injected with BLM at the dose of 1.5 mg/kg to induce pulmonary fibrosis. The IL-11 antagonist IL-11 Rα FC (2.5 mg/kg) was administered via the tail vein to the mice in the IL-11 antagonist group and BLM + IL-11 antagonist group every 3 days from the BLM injection. The survival status of the mice was observed. On the 21st day after modeling, HE staining, Masson staining, and Ashcroft score were used to evaluate the degree of pulmonary fibrosis. The content of hydroxyproline (HYP) in lung tissue was determined by the alkaline hydrolysis method. The gene and protein expressions of Collagen I, Collagen III, and α-SMA in lung tissues were detected by real-time PCR and Western blot, respectively. TGF-β1 content in lung tissue was determined by enzyme-linked immunosorbent assay. Results: Compared with the control group, BLM reduced the survival rate, destructed the lung tissue, and increased the gene and protein expressions of Collagen I, Collagen III, α-SMA, and the content of TGF-β1 in lung tissue. While, IL-11 Rα Fc treatment improved the survival rate of BLM-induced pulmonary mice, reduced pathological changes, and hydroxyproline content in lung tissue. IL-11 Rα Fc also reduced Collagen I, Collagen III, and α-SMA mRNA and protein expression in the lungs of BLM-treated mice, as well as TGF-β1 content. Conclusion: The IL-11 antagonist alleviates BLM-induced pulmonary fibrosis in mice, which provides a new idea for the clinical treatment of pulmonary fibrosis. |
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| Keywords: | pulmonary fibrosis interleukin 11 bleomycin collagen deposition mice |
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