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High Production of Thermostable β-Galactosidase of Bacillus stearothermophilus in Bacillus subtilis |
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| Authors: | Haruhisa Hirata Seiji Negoro Hirosuke Okada |
| Abstract: | By cloning the β-galactosidase gene of Bacillus stearothermophilus IAM11001 (ATCC 8005) into Bacillus subtilis, enzyme production was enhanced 50 times. β-Galactosidase could be purified to 80% homogeneity by incubating the cell extract of B. subtilis at 70°C for 15 min, followed by centrifugation to remove the denatured proteins. Because of its heat stability and ease of production, β-galactosidase is suitable for application in industrial processes. |
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