Isolation of nucleoli and localization of ribonucleic Acid polymerase I from soybean hypocotyl |
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| Authors: | Lin C Y Guilfoyle T J Chen Y M Key J L |
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| Affiliation: | Department of Botany, University of Georgia, Athens, Georgia 30602. |
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| Abstract: | An effective method for the isolation of nucleoli from auxin-treated soybean (Glycine max, var. Wayne) hypocotyl was developed by polytron homogenization and sucrose gradient centrifugation. The nucleoli expressed only the α-amanitin-insensitive RNA synthetic activity. This activity chromatographed as RNA polymerase I on DEAE-cellulose. It appears that the plant nucleolus, like the animal nucleolus, is the site of localization for RNA polymerase I. |
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