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Reproducible Plaquing System for Rabies, Lymphocytic Choriomeningitis, and Other Ribonucleic Acid Viruses in BHK-21 / 13S Agarose Suspensions
Authors:W. D. Sedwick and T. J. Wiktor
Affiliation:1The Wistar Institute of Anatomy and Biology, and the World Health Organization International Reference Centre for Rabies at The Wistar Institute, Philadelphia, Pennsylvania 19104
Abstract:BHK21/13S-agarose suspension cultures provide an excellent plaquing system for rabies and lymphocytic choriomeningitis viruses. The system has facilitated accurate and reproducible assay of serum neutralization and cloning of these viruses. Rubella, reo, and Kern Canyon viruses may also be assayed by this technique.
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