| 外生菌根菌不同接种方法对樟子松苗木生长的影响 |
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| 引用本文： | 宋瑞清,李喜梅,祁金玉. 外生菌根菌不同接种方法对樟子松苗木生长的影响[J]. 植物生态学报, 2008, 32(6): 1378-1385. DOI: 10.3773/j.issn.1005-264x.2008.06.019 |
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| 作者姓名： | 宋瑞清 李喜梅 祁金玉 |
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| 作者单位： | (1 东北林业大学林学院,哈尔滨 150040) ; (2 黑龙江省林业科学院,哈尔滨 150081);(3 沈阳农业大学,沈阳 110161) |
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| 基金项目： | 国家"十一五"攻关专题 |
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| 摘 要： | 以提高外生菌根真菌对樟子松(Pinus sylvestris var. mongolica)苗木促生长效果为目的, 在前期研究的基础上, 采用菌株配对培养的方法对获得的樟子松外生菌根真菌进行混合接种菌株组合筛选; 采用苗木截根-菌液浸根和沟施接种方法分别对2年生和3年生樟子松苗木进行野外单接种及混合接种。研究外生菌根真菌不同接种方法、菌株单接种及混合接种对樟子松苗木生长的影响。试验结果表明: 供试菌株及菌株组合对樟子松苗木生长均有一定的促进作用。菌株GT005和菌株035为供试菌株中对樟子松苗木促生长效果最佳的菌株。采用苗木截根-菌液浸根方法接种2年生樟子松苗木130 d, GT005接种的苗木高生长提高54%, 地径生长提高15%, 过氧化氢酶活性提高48%, 而根系活力降低3%; 菌株035接种的苗木高生长提高42%, 地径生长提高56%, 过氧化氢酶活性提高47%, 根系活力提高11%。沟施接种方法接种3年生樟子松苗木100 d, GT005接种苗木高生长提高10%, 地径生长提高15%, 过氧化氢酶活性提高90%, 而根系活力降低34%; 菌株035接种苗木高生长提高7%, 地径生长提高9%, 过氧化氢酶活性提高6%, 而根系活力降低46%; 菌株组合044/GT001和GT001/GT005 接种的苗木高生长仅比对照提高3.47%和2.07%, 而菌株组合 044/025 和044/009 接种的苗木高生长低于对照; 混合接种的苗木其地径生长高于对照0.16%～7.98%。综上所述, 苗木截根-菌液浸根接种方法对苗木的促生长效果显著高于沟施接种方法; 外生菌根菌高效菌株与一般菌株混合接种会弱化高效菌株自身接种效果; 苗木过氧化氢酶活性、苗木根系活力与苗木的生物量间无相关性。
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| 关 键 词： | 樟子松苗木 外生菌根菌 接种方法 单接种 混合接种 促生长效果 |
EFFECTS OF INOCULATION OF ECTOMYCORRHIZAL FUNGI ON THE SEEDLING GROWTH OF MONGOL SCOTCH PINE (PINUS SYLVESTRIS VAR. MONGOLICA) |
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| SONG Rui-Qing,LI Xi-Mei,QI Jin-Yu. EFFECTS OF INOCULATION OF ECTOMYCORRHIZAL FUNGI ON THE SEEDLING GROWTH OF MONGOL SCOTCH PINE (PINUS SYLVESTRIS VAR. MONGOLICA)[J]. Acta Phytoecologica Sinica, 2008, 32(6): 1378-1385. DOI: 10.3773/j.issn.1005-264x.2008.06.019 |
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| Authors: | SONG Rui-Qing LI Xi-Mei QI Jin-Yu |
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| Affiliation: | 1Northeast Forestry University, Harbin 150040, China; 2Heilongjiang Academy of Forestry Sciences, Harbin 150081, China; 3Shenyang Agricultural University, Shenyang 110161, China |
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| Abstract: | Aims Ectomycorrhizal fungi promote growth of Mongol Scotch pine (Pinus sylvestris var. mongolica) seedlings. Our objective was to determine the effects of different inoculation methods, different combinations of fungal strains and different single strains on growth of Mongol Scotch pine seedlings. Methods We screened combinations of different ectomycorrhizal fungus strains by dual culture method. Two-year-old seedlings were inoculated with different single strains of ectomycorrhizal fungi by soaking roots in the liquid culture of the ectomycorrhizal fungus. Three-year-old seedlings were inoculated with different single strains using lister method in the field. Seedlings inoculated by PD liquid culture medium served as a control. Three-year-old seedlings were inoculated by different combinations of ectomycorrhizal fungus strains using lister method in the field, and those inoculated by different single strains served as a control. Important findings All single strain and their combinations promoted the growth of Mongol Scotch pine seedlings. Strains GT005 and 035 had the largest growth-promoting effects in all combinations and single strain. Compared with the control, average heights of 2-year-old seedlings inoculated by strains GT005 and 035 increased 54% and 42%, respectively, and average collar diameters increased 15% and 56%, respectively. Average heights of 3-year-old seedlings inoculated by strains GT005 and 035 increased 10% and 7%, respectively, and average collar diameters increased 10% and 9%, respectively. The growth-promoting effect of the inoculating method of root soaking is greater than that of the lister inoculating method. When mixed with another strain, the effect of the high-effect strain on growth of seedlings was reduced. The activity of hydrogen peroxidase and root activity are not correlated with seedling biomass. |
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| Keywords: | seedlings of Pinus sylvestris var. mongolica ectomycorrhizal fungi inoculation method single-inoculation mixed-inoculation growth-promoting effect |
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