| 冈比亚按蚊嗅觉结合蛋白候选基因cDNA的克隆、鉴定及其表达型分析 |
| |
| 引用本文： | 李正西,Jing-Jiang Zhou. 冈比亚按蚊嗅觉结合蛋白候选基因cDNA的克隆、鉴定及其表达型分析[J]. 昆虫学报, 2004, 47(4): 417-423 |
| |
| 作者姓名： | 李正西 Jing-Jiang Zhou |
| |
| 作者单位： | 1. 中国农业大学昆虫学系,北京,100094
2. Rothamsted Research,Hertfordshire AL5 2JQ,UK |
| |
| 基金项目： | 英国洛桑国际博士后基金资助项目 ( 2 0 0 2 .10 -2 0 0 3 .10 ) |
| |
| 摘 要： | 疟蚊主要依靠嗅觉发现寄主。非洲疟蚊冈比亚按蚊Anopheles gambiae是一种嗜吸人血的疟疾传播媒介昆虫。该文作者基于其全基因组序列，采用RT-PCR和标准分子克隆技术获得2个嗅觉结合蛋白候选基因agLZ3788和agLZ9988。测序分析结果表明，它们具有嗅觉结合蛋白的标志性结构域。进一步采用半定量RT-PCR技术研究了它们的空间表达型，结果发现它们不但在雌蚊触角中表达，也在其他部位(尤其是蚊虫足部)有强的表达。这一发现说明疟蚊嗅觉结合蛋白可能具有更广的功能，也为进一步重组表达和功能研究提供了重要依据。
|
| 关 键 词： | 疟蚊 冈比亚按蚊 嗅觉结合蛋白 半定量RT-PCR 空间表达型 |
| 文章编号： | 0454-6296(2004)04-0417-07 |
| 修稿时间： | 2003-11-10 |
Cloning, identification and expression profiling of the cDNAs of odorant-binding proteins in the malaria mosquito, Anopheles gambiae |
| |
| LI Zheng-Xi,Jing-Jiang ZHOU. Cloning, identification and expression profiling of the cDNAs of odorant-binding proteins in the malaria mosquito, Anopheles gambiae[J]. Acta Entomologica Sinica, 2004, 47(4): 417-423 |
| |
| Authors: | LI Zheng-Xi Jing-Jiang ZHOU |
| |
| Affiliation: | LI Zheng-Xi~1,Jing-Jiang ZHOU~2 |
| |
| Abstract: | Olfaction plays a major role in host finding and selection of malaria mosquitoes. The African malaria mosquito, Anopheles gambiae is a highly anthropophilic malaria-transmitting vector. Two odorant-binding protein (OBP) candidates(agLZ3788 and agLZ9988) have been identified using RT-PCR and standard molecular cloning techniques, based on its whole genome sequences. Sequencing and analysis showed that these two OBP genes are characterized by typical insect OBP motif. Furthermore, semi-quantitative RT-PCR was employed to study the spatial expression patterns of them, and the results demonstrated that they were not only expressed in female antennae,but also in other tissues, especially in mosquito legs. This finding indicates that mosquito OBPs might have other functions than binding odorants, and thus provides an important scientific basis for further recombinant and functional studies of mosquito OBPs. |
| |
| Keywords: | Malaria mosquito Anopheles gambiae odorant-binding protein semi-quantitative RT-PCR spatial expression patterns |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《昆虫学报》浏览原始摘要信息 |
|
点击此处可从《昆虫学报》下载全文 |
