A preliminary crystallographic investigation of avidin |
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| Authors: | N. M. Green and M. A. Joynson |
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| Affiliation: | National Institute for Medical Research, Mill Hill, London N.W.7, U.K., and Laboratory of Molecular Biophysics, Department of Zoology, University of Oxford, Oxford OX1, 3QU, U.K. |
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| Abstract: | Avidin was crystallized in various forms from 3m-ammonium sulphate or sodium phosphate at pH5.2. X-ray-crystallographic analysis of crystals from phosphate showed them to be orthorhombic, space group C222, containing two molecules per unit cell and with a single subunit as the asymmetric unit. The formation of the complex with biotin did not affect the morphology of the crystals. |
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