Silencing of Toll pathway components by direct injection of double-stranded RNA into Drosophila adult flies |
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| Authors: | Goto Akira Blandin Stéphanie Royet Julien Reichhart Jean-Marc Levashina Elena A |
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| Affiliation: | Akira Goto, Stéphanie Blandin, Julien Royet, Jean-Marc Reichhart, and Elena A. Levashina |
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| Abstract: | Double-stranded RNA (dsRNA) gene interference is an efficient method to silence gene expression in a sequence-specific manner. Here we show that the direct injection of dsRNA can be used in adult Drosophila flies to disrupt function of endogenous genes in vivo. As a proof of principle, we have used this method to silence components of a major signaling cascade, the Toll pathway, which controls fruit fly resistance to fungal and Gram-positive bacterial infections. We demonstrate that the knockout is efficient only if dsRNA is injected in 4- or more day-old flies and that it lasts for at least 1 week. Furthermore, we report dsRNA-based epistatic gene analysis via injection of a mixture of two dsRNAs and propose that injection of dsRNA represents a powerful method for rapid functional analysis of genes in Drosophila melanogaster adults, particularly of those whose mutations are lethal during development. |
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