| 微波诱变结合抗性突变筛选放线菌素D高产菌株 |
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| 引用本文： | 任林英,张祝兰,王徳森,杨煌建,唐文力,孙菲. 微波诱变结合抗性突变筛选放线菌素D高产菌株[J]. 生物技术进展, 2015, 5(5): 377-381. DOI: 10.3969/j.issn.2095-2341.2015.05.09 |
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| 作者姓名： | 任林英 张祝兰 王徳森 杨煌建 唐文力 孙菲 |
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| 作者单位： | 福建省微生物研究所, 福建省新药(微生物)筛选重点实验室, 福州 350007 |
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| 基金项目： | 福建省科技项目(2014R1009-10) 资助。 |
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| 摘 要： | 采用微波结合链霉素抗性筛选法选育放线菌素D的高产菌株。通过考察链霉素对Streptomyces rubiginosohelvolus FIM-N31菌株孢子生长情况的影响确定链霉素致死浓度，出发菌株FIM-N31的孢子经微波辐照处理后，涂布在含链霉素致死浓度（50 μg/mL）的培养基平板上培养，获得了大量的链霉素抗性基因突变株。摇瓶发酵筛选突变株，结果获得一株遗传性状稳定的放线菌素D高产菌Str186，其产放线菌素D的能力比出发菌株提高了8倍以上。
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| 关 键 词： | 链霉菌 放线菌素 D 抗生素抗性筛选 复合诱变 |
| 收稿时间： | 2015-05-08 |
Screening of Actinomycin D High-producing Strain by Streptomycin Resistant Mutant Selection Coupled with Microwave Mutagenesis |
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| REN Lin-ying,ZHANG Zhu-lan,WANG De-sen,YANG Huang-jian,TANG Wen-li,SUN Fei. Screening of Actinomycin D High-producing Strain by Streptomycin Resistant Mutant Selection Coupled with Microwave Mutagenesis[J]. CURRENT BIOTECHNOLOGY, 2015, 5(5): 377-381. DOI: 10.3969/j.issn.2095-2341.2015.05.09 |
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| Authors: | REN Lin-ying ZHANG Zhu-lan WANG De-sen YANG Huang-jian TANG Wen-li SUN Fei |
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| Affiliation: | Fujian Provincial Key Laboratory of Screening for Novel Microbial Products, Fujian Institute of Microbiology, Fuzhou 350007, China |
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| Abstract: | Microwave mutagenesis and streptomycin resistance screening was applied to screen actinomycin D high-producting strain. After testing the resistance of Streptomyce rubiginosohelvolus FIM-N31 to streptomycin, the lethal concentration of streptomycin was determined. A lot of streptomycin-resistant mutants were screened after the spores regenerated on the lethal streptomycin concentration (50 μg/mL) media when they were treated with microwave irradiation. It was eventually found that the high-yield strain Str186 from these mutants which was stably inheritable, and the productivity was beyond eight times higher than the original strains in the rotation-flask experiments. |
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| Keywords: | Streptomyces actinomycin D antibiotics resistance gene screening compound mutation |
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