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Extended gene expression by medium exchange and repeated transient transfection for recombinant protein production enhancement
Authors:Laura Cervera  Sonia Gutiérrez‐Granados  Nicholas Simon Berrow  Maria Mercedes Segura  Francesc Gòdia
Affiliation:1. Grup d'Enginyeria Cellular i Tissular, Escola d'Enginyeria, Universitat Autònoma de Barcelona, Campus de Bellaterra, Cerdanyola del Vallès, Barcelona, Spain;2. Protein Expression Core Facility, Institute for Research in Biomedicine (IRB) Barcelona, Barcelona, Spain;3. bluebird bio, Pharmaceutical Sciences, Process Development group, Cambridge, Massachusetts;4. +34 935814790+34 935812013
Abstract:
Keywords:HEK 293  transient transfection  recombinant proteins  VLPs  production optimization
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