In Vivo and In Vitro Synthesis of Human Rhinovirus Type 2 Ribonucleic Acid |
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| Authors: | F. H. Yin E. Knight Jr. |
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| Affiliation: | aCentral Research Department, Experimental Station, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898 |
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| Abstract: | HeLa cells infected with human rhinovirus type 2 synthesize a mixture of single-and double-stranded ribonucleic acid (RNA). The RNA synthesized by the membrane-bound RNA polymerase complex in vitro is also a mixture of single- and double-stranded RNA, whereas the deoxycholate-treated RNA polymerase complex synthesized only double-stranded RNA. Although twice as much cell-associated viral RNA is synthesized in vivo at 34 C than at 37 C, there is no difference in the rate of RNA synthesized in vitro at 34 C and 37 C by the polymerase complex. The RNA polymerase complex, after treatment with deoxycholate, sediments as a broad peak with an average sedimentation value of 120S. |
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