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Cloning and Expression Analysis of Two Wheat cDNAs Encoding Cinnamoyl-CoA Reductase
Authors:LIN Zhan-Bing  MA Qing-Hu  MA Mi
Abstract:Cinnamoyl-CoA reductase (CCR) is responsible for the first committed reaction in monolignol biosynthesis, which diverts phenylpropanoid-derived metabolites into the biosynthesis of lignin. To gain a better understanding of the lignin biosynthesis in wheat development, two cDNAs encoding CCR were identified from wheat ( Triticum aestivum L. cv. H4564). DNA sequence analyses indicated that the two cDNAs represent two classes of CCR. RT-PCR and Northern blot hybridization demonstrated that one of them, W-cr6, was expressed actively in stem and leaf tissue, the other one, W-cr19, was expressed in root and stem tissue. The results suggested that there are at least two genes encoded for CCR existing in wheat genome.
Keywords:Triticum aestivum    cDNA cloning   cinnamoyl-CoA reductase   lignin  
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