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Morphological study of the reversion to bacillary form, of Bacillus megaterium protoplasts.
Authors:G Hadlaczky, K Fodor,   L Alf  ldi
Affiliation:G Hadlaczky, K Fodor, and L Alföldi
Abstract:Protoplasts of Bacillus megaterium readily reverted to bacillary form in liquid media and when plated in a soft-agar layer onto the surface of appropriate agar media. Three phases of the reversion sequence could be differentiated by phase contrast microscopy: (i) increase in size of the individual protoplasts, (ii) non oriented division of the protoplasts and (iii) outgrowth of the bacillary forms. With time-lapse photomicrography, reversion sequences of single protoplasts were demonstrated.
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