Morphological study of the reversion to bacillary form, of Bacillus megaterium protoplasts. |
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Authors: | G Hadlaczky, K Fodor, L Alf ldi |
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Affiliation: | G Hadlaczky, K Fodor, and L Alföldi |
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Abstract: | Protoplasts of Bacillus megaterium readily reverted to bacillary form in liquid media and when plated in a soft-agar layer onto the surface of appropriate agar media. Three phases of the reversion sequence could be differentiated by phase contrast microscopy: (i) increase in size of the individual protoplasts, (ii) non oriented division of the protoplasts and (iii) outgrowth of the bacillary forms. With time-lapse photomicrography, reversion sequences of single protoplasts were demonstrated. |
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