Allergen‐specific immune response suppresses interleukin 10 expression in B cells via increasing micro‐RNA‐17‐92 cluster |
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| Authors: | Zhi‐Qiang Liu Gui Yang Jiang‐Qi Liu Lu Zeng Xiao‐Xi Li Li‐Hua Mo Zhi‐Gang Liu Ping‐Chang Yang |
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| Affiliation: | 1. ENT Institute of the Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China;2. Longgang ENT Hospital, Shenzhen, China |
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| Abstract: | Interleukin (IL)‐10–expressing B cells play a critical role in the immune homeostasis in the body; its regulation has not been fully understood. Micro‐RNA (miR)‐17‐92 cluster has strong regulation in the immunity. This study tests a hypothesis that miR‐17‐92 cluster suppresses IL‐10 expression in B cells. In this study, peripheral B cells were collected from patients with allergic rhinitis (AR). The B cells were treated with specific allergens, dust mite extracts, in the culture. The expressions of miR‐17‐92 cluster and IL‐10 in the culture were assessed by real‐time quantitative reverse transcription polymerase chain reaction and enzyme‐linked immunosorbent assay. The results showed that the levels of miR‐19a, but not the rest of the 5 members (miR‐17, miR‐18a, miR‐19b, miR‐20a, and miR‐92a), were significantly higher in peripheral B cells from AR patients as than in B cells from healthy participants. Exposure of B cells from AR patients to specific allergen, dust mite extracts, significantly increased the levels if miR‐19a and suppressed the expression of IL‐10 in B cells. The levels of histone deacetylase 11 and acetylated H3K9 were higher, and the RNA polymerase II and c‐Maf (the IL‐10 transcription factor) were lower, at the IL‐10 promoter locus. In conclusion, miR‐19a mediates the allergen‐specific immune response–decreased IL‐10 expression in B cells. |
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| Keywords: | allergy B cell interleukin‐10 micro‐RNA mite allergen |
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