Efficiency of particle-bombardment-mediated transformation is influenced by cell cycle stage in synchronized cultured cells of tobacco |
| |
| Authors: | Iida A Yamashita T Yamada Y Morikawa H |
| |
| Affiliation: | Sumitomo Chemical Co., Ltd., Biotechnology Laboratory, Takarazuka Research Center, 4-2-1 Takatsukasa, Takarazuka, Hyogo 665, Japan. |
| |
| Abstract: | Plasmid DNA pB1221 harboring β-glucuronidase gene was delivered to synchronized cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells of different cell cycle stages by a pneumatic particle gun. The cells bombarded at M and G2 phases gave 4 to 6 times higher transformation efficiency than those bombarded at the S and G1 phases. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
