Identification of DNA Segments Capable of Rescuing a Non-Mendelian Mutant in Paramecium |
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| Authors: | C. S. Kim J. R. Preer-Jr B. Polisky |
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| Affiliation: | Program in Molecular, Cellular, and Developmental Biology, Department of Biology, Indiana University, Bloomington, Indiana 47405 |
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| Abstract: | The non-Mendelian mutant d48 of Paramecium tetraurelia contains micronuclear wild type A genes, but at autogamy and conjugation proper processing fails and new macronuclei lack A genes. When cloned A genes are injected into the macronucleus of d48, proper processing is restored at the next autogamy; d48 is rescued, becoming permanently wild type. In the present study we have injected portions of the A gene into d48. We find that the ability to rescue extends over a large portion of the gene, with highest activity near a series of 221-bp repeat units in the middle of the gene. Regions outside the A gene are inactive. |
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