A protected biotin containing deoxycytidine building block for solid phase synthesis of biotinylated oligonucleotides. |
| |
| Authors: | U Pieles B S Sproat G M Lamm |
| |
| Affiliation: | European Molecular Biology Laboratory, Heidelberg, FRG. |
| |
| Abstract: | The synthesis of a modified 2'-deoxycytidine-3'-O-phosphoramidite carrying an N-t-butylbenzoyl protected biotin on a long polar spacer arm attached to the 4-N position is described. The presence of the bulky lipophilic t-butylbenzoyl protecting group enables the direct solid phase synthesis of biotinylated oligoribonucleotides and a variety of analogues in high yield without modification of the biotin moiety. Biotinylated antisense oligonucleotides incorporating this new derivative allow convenient isolation and purification of ribonucleic acid-protein complexes. The kinetics of biotin binding to streptavidin agarose is facilitated by the long polar spacer arm. |
| |
| Keywords: | |
|
