Respiration-driven proton translocation in Escherichia coli (Short Communication) |
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| Authors: | Hugh G. Lawford and Bruce A. Haddock |
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| Affiliation: | Department of Biochemistry, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, U.K. |
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| Abstract: | Measurements were made of the stoicheiometry of respiration-driven proton translocation coupled to the oxidation of NAD(P)-linked or flavin-linked substrates in intact cells of Escherichia coli. Observed stoicheiometries (-->H(+)/O quotient; Mitchell, 1966) were approx. 4 with l-malate as substrate and approx. 2 for succinate, d-lactate and glycerol oxidation. It is concluded that the potential number of equivalent energy-conservation sites associated with the respiratory chain is 2 in aerobically grown cells of E. coli harvested during the exponential phase of growth. |
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