| 家蚕雌激素相关受体基因的克隆与表达分析 |
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| 引用本文： | 沈关望,胡诗圆,王勇,吴金鑫,林英,夏庆友. 家蚕雌激素相关受体基因的克隆与表达分析[J]. 昆虫学报, 2015, 58(12): 1278-1284 |
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| 作者姓名： | 沈关望 胡诗圆 王勇 吴金鑫 林英 夏庆友 |
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| 作者单位： | (1. 西南大学家蚕基因组生物学国家重点实验室, 重庆 400716; 2. 西南大学生物技术学院, 重庆 400716） |
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| 摘 要： | 【目的】黑腹果蝇 Drosophila melanogaster 雌激素相关受体（estrogen-related receptor, ERR）通过调节糖酵解过程进而控制果蝇的能量代谢。本研究在克隆家蚕Bombyx mori ERR 基因 (BmERR) 的基础上，对其分子特性和系统演化进行生物信息学分析, 并检测该基因在家蚕生殖腺中的表达，为进一步研究ERR功能奠定基础。【方法】采用PCR技术克隆 BmERR 基因的全长cDNA序列，进行生物信息学分析；利用半定量RT-PCR检测该基因在停食后家蚕幼虫生殖腺中的表达情况。【结果】BmERR 基因全长cDNA序列为1 296 bp，编码431个氨基酸残基；具有ERR蛋白家族典型的结构特征；系统进化分析显示BmERR与其他昆虫ERR氨基酸序列一致性较高；半定量 RT-PCR 检测表明，BmERR 在家蚕上簇到化蛾期间的精巢和卵巢中均有表达，表达具有时期特异性，化蛹第1天达到表达高峰。【结论】本研究首次从鳞翅目昆虫中克隆获得ERR cDNA序列。ERR基因在家蚕生殖腺中表达量无明显性别差异，但具有发育时期特异性。
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| 关 键 词： | 家蚕 雌激素相关受体 克隆 序列分析 表达谱 |
Molecular cloning and expression profiling of an estrogen-related receptor gene in the silkworm,Bombyx mori |
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| SHEN Guan-Wang,HU Shi-Yuan,WANG Yong,WU Jin-Xin,LIN Ying,XIA Qing-You. Molecular cloning and expression profiling of an estrogen-related receptor gene in the silkworm,Bombyx mori[J]. Acta Entomologica Sinica, 2015, 58(12): 1278-1284 |
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| Authors: | SHEN Guan-Wang HU Shi-Yuan WANG Yong WU Jin-Xin LIN Ying XIA Qing-You |
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| Affiliation: | (1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; 2. College of Biotechnology, Southwest University, Chongqing 400716, China） |
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| Abstract: | 【Aim】 The estrogen-related receptor (ERR) via regulating the glycolytic cyclecan control the energy metabolism in Drosophila melanogaster. Based on the cloning of ERR gene (BmERR) from the silkworm, Bombyx mori, its molecular property and phylogeny were bioinformatically analyzed, and the expression of the ERR gene in the silkworm was detected. This study may lay the foundation for further research on the function of ERR in Lepidoptera and provide ideas for pest control. 【Methods】 PCR technique was used to clone the cDNA sequence of BmERR gene, and the sequence was bioinformatically analyzed with online resources. The semi-quantitative RT-PCR was further applied to analyze its expression profiles in gonads of B. mori larvae stopping feeding. 【Results】 The full-length cDNA of BmERR has a 1 296 bp open reading frame, which encodes a 431-amino-acid-residue protein with a typical structural domain feature of the ERR protein family. Phylogenetic analysis indicated that the BmERR protein shows high amino acid sequence identity with the ERR proteins from other insects. RT-PCR detection showed that BmERR was expressed in both testis and ovary in wandering to pupal B. mori, and possessed apparent time specificity, with a peak expression at the first day after pupation. 【Conclusion】 The cloned ERR encoding sequence is reported for the first time in Lepidoptera. The expression of the ERR gene in the silkworm gonads has no significant gender difference, but with developmental stage specificity. |
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| Keywords: | Bombyx mori estrogen-related receptor cloning sequence analysis expression profile |
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