Molecular Analysis of an Unstable P Element Insertion at the Singed Locus of Drosophila Melanogaster: Evidence for Intracistronic Transposition of a P Element |
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| Authors: | R. S. Hawley R. A. Steuber C. H. Marcus R. Sohn D. M. Baronas M. L. Cameron A. E. Zitron J. W. Chase |
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| Affiliation: | Department of Genetics, Albert Einstein College of Medicine, Bronx, New York 10461. |
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| Abstract: | In a companion study, a number of P element insertions into the singed locus were characterized. Here is reported a detailed analysis of the structure and mutability of another P element insertion at sn, known as sncm. Under conditions which mobilize P elements, sncm mutates at high frequency to both wild-type (sn+) and to a much more extreme allele (snext). Wild-type revertants appear to represent precise or nearly precise excisions of the P element. Certainly two, and most likely all five, of the snext alleles studied result from the insertion of a duplicate copy of this P element into a nearby site in an inverted orientation. We propose a model in which both the sn+ and snext mutational events can be explained by excision of the P element from one chromatid followed by reintegration into the sister chromatid at a nearby site (intracistronic transposition). Finally, it is shown that the snext alleles are themselves unstable and the structure of a resulting chromosome aberration is examined. |
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