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| 悬浮培养南方红豆杉细胞的紫杉醇生物合成路径中诱导子的作用位点 | |
| 引用本文： | 苗志奇,元英进,任德锋,未作君,吴金川. 悬浮培养南方红豆杉细胞的紫杉醇生物合成路径中诱导子的作用位点[J]. 植物学报(英文版), 2004, 46(6): 730-737 |
| 作者姓名： | 苗志奇 元英进 任德锋 未作君 吴金川 |
| 作者单位： | 苗志奇 (天津大学化工学院制药工程系,天津,300072) ； 元英进 (天津大学化工学院制药工程系,天津,300072) ； 任德锋 (天津大学化工学院制药工程系,天津,300072) ； 未作君 (天津大学化工学院制药工程系,天津,300072) ； 吴金川 (天津大学化工学院制药工程系,天津,300072) ； |
| 摘 要： | 作者研究开发出一种基于分析中间响应模式确定悬浮培养诱导子作用位点的新方法.研究结果表明,一个诱导子的作用位点存在于浓度变化方向相反的相邻两个中间代谢物之间;该方法的有效性在悬浮培养南方红豆杉(Taxus chinensis(Pilg.)Rehd.var.mairei(Lemee et Levl.)Cheng et L. K.Fu)生物合成紫杉醇过程中得以证实;经确定,甲基茉莉酮酸、硝酸根和柠檬酸铵的作用位点在baccatinⅢ至10-去乙酰基紫杉醇之间;水杉酸、花生四烯酸的作用位点存在3种可能性,即增强10-去乙酰基紫杉醇的合成、防止紫杉醇和cephalomannine的降解.该方法对指导诱导子配伍优化紫杉醇生产具有指导意义. |
| 关 键 词： | 作用路径 诱导子 中间响应模式 悬浮培养 紫杉醇 南方红豆杉 |
Acting Point in Taxol Biosynthesis Pathway of Elicitor in Suspension Cultures of Taxus chinensis var.mairei |
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| MIAO Zhi-Qi,YUAN Ying-Jin,REN De-Feng,WEI Zuo-Jun,WU Jin-Chuan. Acting Point in Taxol Biosynthesis Pathway of Elicitor in Suspension Cultures of Taxus chinensis var.mairei[J]. Journal of integrative plant biology, 2004, 46(6): 730-737 | |
| Authors: | MIAO Zhi-Qi YUAN Ying-Jin REN De-Feng WEI Zuo-Jun WU Jin-Chuan |
| Abstract: | A new method was developed to determine the acting paths of elicitors based on the analysis of intermediate response mode in suspension cultures. It was found that the acting path of an elicitor, defined as the biosynthesis step whose rate was significantly enhanced upon the addition of the elicitor, was located between two adjacent intermediates whose concentrations changed in opposite directions. The validity of this method was verified in the biosynthesis of taxol from suspension cultures of Taxus chinensis (Pilg.) Rehd. var. mairei (Lemee et Levl.) Cheng et L. K. Fu. The acting paths of methyl jasmonate, silver nitrate and ammonium citrate were determined to be located at the step from baccatin Ⅲ to 10-deacyl taxol. There are three possibilities for salicylic acid and arachidonic acid: (1) improving the biosynthesis of 10-deactyl baccatin Ⅲ (10-DAB), (2) inhibiting the degradation of taxol and (3) inhibiting the degradation of cephalomannine. But it is difficult to elucidate the exact acting paths of these two elicitors based on the present method. |
| Keywords: | acting path elicitor,intermediate response mode suspension culture taxol Taxus chinensis var. mairei |
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