Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1 |
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| Authors: | Blondal Thorarinn Hjorleifsdottir Sigridur H Fridjonsson Olafur F Aevarsson Arnthor Skirnisdottir Sigurlaug Hermannsdottir Anna Gudny Hreggvidsson Gudmundur O Smith Albert Vernon Kristjansson Jakob K |
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| Affiliation: | Thorarinn Blondal, Sigridur H. Hjorleifsdottir, Olafur F. Fridjonsson, Arnthor Ævarsson, Sigurlaug Skirnisdottir, Anna Gudny Hermannsdottir, Gudmundur O. Hreggvidsson, Albert Vernon Smith, and Jakob K. Kristjansson |
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| Abstract: | Thermophilic viruses represent a novel source of genetic material and enzymes with great potential for use in biotechnology. We have isolated a number of thermophilic viruses from geothermal areas in Iceland, and by combining high throughput genome sequencing and state of the art bioinformatics we have identified a number of genes with potential use in biotechnology. We have also demonstrated the existence of thermostable counterparts of previously known bacteriophage enzymes. Here we describe a thermostable RNA ligase 1 from the thermophilic bacteriophage RM378 that infects the thermophilic eubacterium Rhodothermus marinus. The RM378 RNA ligase 1 has a temperature optimum of 60–64°C and it ligates both RNA and single-stranded DNA. Its thermostability and ability to work under conditions of high temperature where nucleic acid secondary structures are removed makes it an ideal enzyme for RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE), and other RNA and DNA ligation applications. |
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