Efficiency of homologous DNA recombination varies along the Bacillus subtilis chromosome. |
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| Authors: | V Vagner and S D Ehrlich |
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| Affiliation: | Institut des Biotechnologies, Centre de Recherche de Jouy en Josas, France. |
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| Abstract: | Structures consisting of a genetic marker (erythromycin or kanamycin resistance, thymidylate synthetase) flanked by 3.4-kilobase direct repeats (pBR322 sequences) were inserted in 12 different locations of the Bacillus subtilis chromosome. Recombination between the repeats was followed by the loss of the genetic marker. Recombination frequencies found in different locations varied from 1.2 X 10(-5) to 40 X 10(-5) per cell generation. Such differences were highly significant (P less than 0.001). |
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