| 硝酸盐对球形棕囊藻生长和硝酸还原酶活性的影响 |
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| 引用本文： | 王艳 唐海溶 蒋磊 李韶山. 硝酸盐对球形棕囊藻生长和硝酸还原酶活性的影响[J]. 植物学报, 2006, 23(2): 138-144 |
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| 作者姓名： | 王艳 唐海溶 蒋磊 李韶山 |
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| 作者单位： | (1 暨南大学理工学院环境工程系 广州 510632) (2 暨南大学生命科学学院 广州 510632) (3 华南师范大学生命科学学院 广州 510631) |
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| 基金项目： | 中国科学院资助项目,科技部科研项目,国家留学回国人员科研启动基金,广东省教育厅自然科学基金 |
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| 摘 要： | 以我国南海海域分离的赤潮原因种——球形棕囊藻(Phaeocystis globosa)为材料, 研究了不同硝酸盐浓度下藻细胞生长及硝酸还原酶活性的变化。当培养基中不含硝酸盐时, 藻细胞内硝酸还原酶的活性保持在非常低的水平, 藻细胞的生长受到限制, 不能形成正常的生长曲线: 当培养基中硝酸盐浓度为3.62 mmol.L-1时, 藻细胞的硝酸还原酶活性和比生长速率达到最大。在含有硝酸盐的培养基中, 接种培养后第9天藻细胞硝酸还原酶活性达到最大值, 并且在4种不同硝酸盐浓度下, 藻细胞硝酸还原酶活性的差异性达到极显著水平(P<0.01)。在接种培养第16天藻细胞密度达到最大值, 并且4种不同硝酸盐浓度培养的藻细胞密度之间的差异性也达到极显著水平(P<0.01)。实验结果表明, 在培养基中添加不同浓度的硝酸盐, 对球形棕囊藻细胞硝酸还原酶的活性和藻细胞的生长有极显著的影响, 含有较高硝酸盐的富营养化海域有利于球形棕囊藻细胞的持续生长。
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| 关 键 词： | 球形棕囊藻 硝酸还原酶 硝酸盐 有害赤潮 |
| 收稿时间： | 2005-07-06 |
| 修稿时间： | 2005-12-05 |
Effects of Nitrate on the Growth and Nitrate Reductase Activity in Phaeocystis globosa |
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| Yan Wang,Hairong Tang,Lei Jiang,Shaoshan Li. Effects of Nitrate on the Growth and Nitrate Reductase Activity in Phaeocystis globosa[J]. Bulletin of Botany, 2006, 23(2): 138-144 |
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| Authors: | Yan Wang Hairong Tang Lei Jiang Shaoshan Li |
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| Affiliation: | (1 Department of Environmental Engineering, College of Science & Engineering, Jinan University,Guangzhou 510632) (2 College of Life Science, Jinan University, Guangzhou 510632) (3 College of Life Science, South China Normal University, Guangzhou 510631) |
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| Abstract: | Phaeocystis globosa, which causes harmful algae bloom (HAB), was isolated from the South China Sea and grown in modified F/2 medium under varying nitrate concentrations. Cell growth and nitrate reductase (NR) activity were investigated. P. globosa grown without nitrate showed low-level NR activity, inhibited algal cell growth and no growth curve. The optimal nitrate concentration for algal cell growth and NR activity was 3.62 mmol.L-1. Under 4 different levels of nitrate, NR activity in cells peaked at day 9, but cell density peaked at day 16. Cell density and NR activity differed significantly nitrate concentration. Thus, eutrophic sea water with a high nitrate concentration is suitable for the growth of P. globosa and has great potential for developing into red tide. |
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| Keywords: | ; |
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