| 长链非编码RNA SPRY4-IT1对髓母细胞瘤增殖及侵袭转移的影响 |
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| 引用本文： | 史鹏飞,吉海龙,罗永康,毛天明,陈茜,周开宇. 长链非编码RNA SPRY4-IT1对髓母细胞瘤增殖及侵袭转移的影响[J]. 中国应用生理学杂志, 2017, 33(1): 78-82. DOI: 10.12047/j.cjap.5448.2017.020 |
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| 作者姓名： | 史鹏飞 吉海龙 罗永康 毛天明 陈茜 周开宇 |
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| 作者单位： | 1. 温州医科大学附属第一临床医学院, 浙江温州 325000;2. 浙江省台州市立医院神经外科, 台州 318000;3. 浙江省台州市立医院病理科, 台州 318000 |
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| 基金项目： | 浙江省中医药管理局科技计划资助项目（2013ZA133，2015ZB133）；台州市科技局科技计划资助项目（14SF05） |
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| 摘 要： | 目的：探讨长链非编码RNA SPRY4-IT1（LncRNA）对髓母细胞瘤增殖及侵袭转移的影响。方法：髓母细胞瘤Daoy细胞分为对照组和si-SPRY4-IT1组，分别利用脂质体Lipofectamine 2000将阴性对照荧光序列和SPRY4-IT1-siRNA转入细胞中，采用Real-time PCR检测转染后各组细胞中SPRY4-IT1的表达情况，CCK-8实验及平板克隆形成实验检测细胞增殖能力的变化，以细胞体外侵袭、迁移实验分别检测细胞侵袭、迁移能力的变化，Western blot检测SPRY4-IT1对基质金属蛋白酶（MMP）-2和MMP-9蛋白的影响。结果：si-SPRY4-IT1组SPRY4-IT1 mRNA表达水平、细胞体外增殖能力、细胞侵袭、迁移能力、细胞MMP-2蛋白表达均较对照组明显降低（P<0.05），而MMP-9蛋白表达未见明显变化。结论：干扰长链非编码RNA SPRY4-IT1在髓母细胞瘤Daoy细胞中的表达能显著抑制细胞的增殖、侵袭和迁移能力。
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| 关 键 词： | 髓母细胞瘤 长链非编码RNA(LncRNA) SPRY4-IT1 增殖 侵袭迁移 |
| 收稿时间： | 2016-03-28 |
Effect of long noncoding RNA SPRY4-IT1 on proliferation and metastasis of medulloblastoma |
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| SHI Peng-fei,JI Hai-long,LUO Yong-kang,MAO Tian-ming,CHEN Xi,ZHOU Kai-yu. Effect of long noncoding RNA SPRY4-IT1 on proliferation and metastasis of medulloblastoma[J]. Chinese journal of applied physiology, 2017, 33(1): 78-82. DOI: 10.12047/j.cjap.5448.2017.020 |
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| Authors: | SHI Peng-fei JI Hai-long LUO Yong-kang MAO Tian-ming CHEN Xi ZHOU Kai-yu |
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| Affiliation: | 1. the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000;2. Department of Neurosurgery, Zhejiang Taizhou Municipal Hospital, Taizhou 318000, China;3. Department of Pathology, Zhejiang Taizhou Municipal Hospital, Taizhou 318000, China |
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| Abstract: | Objective: To investigate the effects of long non-coding RNA SPRY4-IT1 (LncRNA) on proliferation and metastasis of medul-loblastoma cells.Methods: SPRY4-IT1siRNA and control fluorescence siRNA were transfected into medulloblastoma cell line Daoy with Lipo-fe ctamine 2000 and were divided into control group and si-SPRY4-IT1 group. Relative expression of SPRY4-IT1 mRNA were detected by real-time quantitative PCR. The change of cell proliferation were examined using CCK-8 kit and clone forming experiment. The change of cell inva-sion and metastasis were examined by matrigel invasion assay and cell metastasis experiment respectively, The expression of matrix metallopro-teinase MMP-2 and MMP-9 were detected by Western blot.Results: In si-SPRY4-IT1 group,the SPRY4-IT1 mRNA expression level, cell pro-liferation in vitro,cell invasion and migration ability, MMP-2 protein expression were significantly lower than those in the control group.Conclusion: Interference SPRY4-IT1 expression has prominent inhibitory effect on the cell proliferation、invasion and metastasis of medulloblastoma cell line Daoy. |
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| Keywords: | medulloblastoma long noncoding RNA (LncRNA) SPRY4-IT1 proliferation invasion and metastasis |
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