| 抗寒剂CR-4 对冬小麦幼苗质膜钙泵（Ca2 +-ATPase） 的稳定作用 |
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| 引用本文： | 孙德兰 王红 简令成. 抗寒剂CR-4 对冬小麦幼苗质膜钙泵（Ca2 +-ATPase） 的稳定作用[J]. 植物学报, 1998, 15(2): 50-54 |
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| 作者姓名： | 孙德兰 王红 简令成 |
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| 作者单位： | (中国科学院植物研究所 北京 100093) |
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| 摘 要： | 通过磷酸铈沉淀的细胞化学观察揭示，常温下生长的冬小麦幼苗的Ca2+ -ATP酶活性主要定位在质膜上，同时，水浸种和抗寒剂浸种的小麦质膜Ca2+ -ATP酶活性没有差异。然而，小麦幼苗经-7℃冰冻处理12小时和24小时后，则表现明显的区别：水浸种的小麦幼苗质膜Ca2+ -ATP酶活性明显下降，直至完全失活，细胞的精细结构也同时被破坏；而经抗寒剂浸种的小麦幼苗质膜Ca2+ -ATP酶仍维持较高的活性，细胞结构也保持完整，显示抗寒剂对质膜Ca2+ -ATPase酶起着明显的稳定作用。
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| 关 键 词： | Ca2+ -ATP 质膜 小麦 低温逆境 抗寒剂CR-4 |
The Stabilizaton on the Plasmalemma Calcium-Pump( Ca2 + -ATPase) in Winter Wheat Seedlings by the Cold-Resistant Agent CR-4 |
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| SUN De-Lan,WANG Hong and JIAN Ling-Cheng. The Stabilizaton on the Plasmalemma Calcium-Pump( Ca2 + -ATPase) in Winter Wheat Seedlings by the Cold-Resistant Agent CR-4[J]. Bulletin of Botany, 1998, 15(2): 50-54 |
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| Authors: | SUN De-Lan WANG Hong JIAN Ling-Cheng |
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| Affiliation: | (Institute of Botany, The Chinese Academy of Science, Beijing 100093) |
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| Abstract: | The electron microscopical observations using Cytochemical method of cerium phosphate precipitation indicated that the Ca2+-ATPase activity was mainly localized at the plasmalemma in wheat seedling cells grown at normal temperature. And the enzyme activity did not appear to be different between water-soaked and CR4-Soaked treatments under normal temperature. However, the differences occurred when wheat seedlings were treated for 12 hrs and 24 hrs at - 7℃: the plasmalemma Ca2+-ATPase activity in the seedlings from water-soaked seeds was markedly decreased, and the ultrastructures were disrupted, and that from cold-resistant agent CR4-Soakod seeds was still maintained, and their free structures were also intact. The results suggest that the cold-resistant agent CR-4 plays an important role for stabilizing plasmalemma calcium pump (Ca2+-ATPase) under low temperature stress. |
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| Keywords: | ; |
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