Antioxidant Capacity,Phenolic Constituents and Toxicity of Hot Water Extract from Red Maple Buds |
| |
| Authors: | Naamwin R. Meda Patrice E. Poubelle Tatjana Stevanovic |
| |
| Affiliation: | 1. Département des Sciences du Bois et de la Forêt, Faculté de Foresterie et Géomatique, Centre de Recherche sur les Matériaux Renouvelables (CRMR), Université Laval, Québec, QC, Canada;2. Institut des Nutraceutiques et des Aliments Fonctionnels (INAF), Université Laval, Québec, QC, Canada;3. Centre de Recherche sur les Matériaux Avancés (CERMA), Université Laval, Québec, QC, Canada;4. Département de Médecine, Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du CHU de Québec, Université Laval, Québec, QC, Canada |
| |
| Abstract: | The present study reports, for the first time, the results of the antioxidant capacity and the phenolic composition of a hot water extract from red maple buds (RMB), as well as its safety. In this regard and comparatively to antioxidant standards, this extract exhibits a significant antiradical capacity when tested by 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH·) and anion superoxide trapping assays. High‐resolution mass spectrometric and nuclear magnetic resonance analyses permitted to determine for the first time, in red maple species, cyanidin‐3‐O‐glucoside, quercetin‐3‐O‐galactoside, quercetin‐3‐O‐arabinoside, and quercetin. Also, the quantification of individual phenolics by high‐performance liquid chromatography method revealed that ginnalin A at 117.0 mg/g is the major compound of RMB hot water extract. Finally, using flow cytometry evaluation, the extract of RMB was determined to have no toxicity neither to cause significant modification of apoptosis process, up to concentration of 100 μg/ml, on human peripheral blood neutrophils. These results allow anticipating various fields of application of RMB water extract. |
| |
| Keywords: | Acer rubrum L. Maple buds Antiradical capacity Phenolic compounds Neutrophil viability |
|
|
