Mutation at the "exit gate" of the salmonella gyrase a subunit suppresses a defect in the gyrase B subunit |
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| Authors: | Blanc-Potard Anne-Béatrice Labesse Gilles Figueroa-Bossi Nara Bossi Lionello |
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| Affiliation: | Avenir team Institut National de la Santé et de la Recherche Médicale, Unité 431, Faculté de Médecine, CS 83021, Avenue J. F. Kennedy, 30908 N?mes Cedex 02, France. ablancpotard@univ-montp1.fr |
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| Abstract: | In Salmonella enterica serovar Typhimurium, an S431P substitution in the B subunit of gyrase (allele gyrB651) confers resistance to nalidixic acid and causes reduced DNA superhelicity and hypersensitivity to novobiocin. Selection for novobiocin resistance allowed isolation of a mutation in the gyrA gene (allele gyrA659), a T467S substitution, which partially suppresses the supercoiling defect of gyrB651. Modeling analysis suggests that this mutation acts by destabilizing the GyrA bottom dimer interface. This is the first example of a gyrA mutation that compensates for a gyrB defect. |
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