The effect of light on the tricarboxylic Acid cycle in green leaves: I. Relative rates of the cycle in the dark and the light |
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| Authors: | Chapman E A Graham D |
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| Affiliation: | Commonwealth Scientific and Industrial Research Organization, Division of Food Research, and School of Biological Sciences, Macquarie University, North Ryde 2113, Sydney, Australia. |
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| Abstract: | Excised green leaves of mung bean (Phaseolus aureus L. var. Mungo) were used to determine the effect of light on the rate of endogenous respiration via the tricarboxylic acid cycle. Illumination with white light at an intensity of 0.043 gram calories cm−2min−1 (approximately 8600 lux) of visible radiation (400-700 nm) gave a rate of apparent photosynthesis, measured as net CO2 uptake, of 21 mg CO2 dm−2hr−1 which was about 11-fold greater than the rate of dark respiration. The feeding of 14CO2 or 14C-labeled acids of the tricarboxylic acid cycle in the dark for 2 hours was established as a suitable method for labeling mitochondrial pools of cycle intermediates. |
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