| 基于iTRAQ技术分析五倍子作用白念珠菌后的差异蛋白表达 |
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| 引用本文： | 张晓龙,李立平,鲁仁义,阎澜,姜远英. 基于iTRAQ技术分析五倍子作用白念珠菌后的差异蛋白表达[J]. 生物技术进展, 2018, 8(6): 537-545. DOI: 10.19586/j.2095-2341.2018.0086 |
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| 作者姓名： | 张晓龙 李立平 鲁仁义 阎澜 姜远英 |
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| 作者单位： | 1.中国人民解放军海军军医大学药学院新药研究中心, 上海 200433;2.同济大学医学院, 上海 200092 |
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| 基金项目： | 国家自然科学基金项目（81573473）资助。 |
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| 摘 要： | 为了探讨五倍子抗白念珠菌的作用机制，提取4 μg/mL五倍子提取液作用后的白念珠菌（SC5314）总蛋白，采用同位素标记相对和绝对定量（isobaric tags for relative and absolute quantitation，iTRAQ）蛋白质组学技术、液相色谱串联质谱（LC-MS/MS）技术分析和鉴定差异表达的蛋白质，并对差异表达蛋白进行生物信息学分析。经LC-MS/MS鉴定出3 721种蛋白质，其中，差异表达蛋白104种，包括57种表达上调蛋白和47种表达下调蛋白。通过生物信息学分析发现，上述差异蛋白参与了氧化还原反应、过氧化氢分解代谢以及能量代谢等生物学过程。研究表明，五倍子抗白念珠菌的作用机制可能是通过抑制氧化磷酸化，进而影响菌体能量代谢和物质生成，最终导致细胞结构与功能的改变。
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| 关 键 词： | 五倍子 白念珠菌 差异蛋白 同位素标记相对和绝对定量 质谱 |
| 收稿时间： | 2018-08-09 |
Analysis of Differential Expression Proteins in Candida albicans Treated with Galla Chinensis by iTRAQ |
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| ZHANG Xiaolong,LI Liping,LU Renyi,YAN Lan,JIANG Yuanying. Analysis of Differential Expression Proteins in Candida albicans Treated with Galla Chinensis by iTRAQ[J]. CURRENT BIOTECHNOLOGY, 2018, 8(6): 537-545. DOI: 10.19586/j.2095-2341.2018.0086 |
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| Authors: | ZHANG Xiaolong LI Liping LU Renyi YAN Lan JIANG Yuanying |
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| Affiliation: | 1.Center for New Drug Research, School of Pharmacy, Naval Medical University, Shanghai 200433, China; 2.School of Medicine, Tongji University, Shanghai 200092, China |
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| Abstract: | In order to explore antifungal mechanism of Galla Chinensis against Candida albicans, the total proteins of Candida albicans (SC5314) treated with 4 μg/mL Galla Chinensis extract were extracted, and isobaric tags for relative and absolute quantization (iTRAQ) labeling and LC-MS/MS were used to analyse and identify differentially expressed proteins, and then the functions of the proteins were analyzed by bioinformatics. A total of 3 721 proteins were identified by LC-MS/MS. Among them, there were 104 differentially expressed proteins, with 57 proteins significantly up-regulated and 47 down-regulated. Through bioinformatics analysis, these differential proteins were involved in biological processes such as redox reactions, hydrogen peroxide catabolism, and energy metabolism process and so on. The results of this research indicated that the antifungal mechanism of Galla Chinensis against Candida albicans might be achieved by inhibiting oxidative phosphorylation, affecting cell energy metabolism and substance production, eventually resulting in changes in cell structure and function. |
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| Keywords: | Galla Chinensis Candida albicans differential protein isobaric tags for relative and absolute quantitation mass spectrum |
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